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bca protein quantitation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bca protein quantitation kit
    Bca Protein Quantitation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+protein+quantitation+kit/BCA+Protein+Assay+Kit/pmc13093712-64-42-46
    Average 99 stars, based on 1 article reviews
    bca protein quantitation kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Protein Quantitation:

    Article Title: The mechanisms by which hypothalamic neuroinflammation induced by neonatal cerebral ischemia–hypoxia leads to decreased thymic function via the HPA axis
    Article Snippet: .. Following the instructions of the BCA protein quantitation kit (Thermo, USA), the apoptosis-promoting proteins Cleaved-Caspase 3 (WL02117, Wanleibio), Bcl-2 (WL01556, Wanleibio) and Bax (WL01637, Wanleibio) in thymus were detected. .. Autophagic proteins Beclin-1 (66665-1, Proteintech), LC3 II/I (2775 S, CST), p-PI3K (ab302958, Abcam), PI3K (4257S, CST), p-Akt (WLP001a, Wanleibio), Akt (4060S, CST), p-mTOR (5536 S, CST), mTOR (2983, CST), GR (WL02695, Wanleibio), β-actin (sc-47778, Santa Cruz) and Histone H3 (4499, CST) in thymus were detected.

    Article Title: Purified Diets Lacking Fermentable Fiber Reduce Microbial Diversity, Alter Epithelial Transcriptome, and Exacerbate Colitis
    Article Snippet: .. Total protein concentrations were measured using the Pierce BCA Protein Quantitation kit (Thermo Fisher Scientific). .. Capillary Western analyses were performed using a Wes system (Protein Simple, San Jose, CA, USA) according to the manufacturer’s instructions.

    Article Title: SYNGR3 Accelerates α‐Synuclein Aggregation and Neurodegeneration in Parkinson's Disease
    Article Snippet: Pellets were washed in 1 mL TBS, centrifuged at 100,000 × g at 4°C for 1 h, subsequently resuspended in 0.25 mL of 2% SDS in TBS, and finally sonicated and centrifuged at 100,000 × g at 4°C for 1 h. Mouse brain tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer (Beyotime, P0013B) supplemented with a protease inhibitor cocktail (Sigma Aldrich, P9599) and phosphatase inhibitor (APExBIO, K1015). .. The homogenates were subsequently centrifuged at 100,000 × g at 4°C for 1.5 h. The pellet was subsequently resuspended in 2% SDS in TBS buffer and centrifuged at 100,000 × g at 4°C for 1 h. Protein levels were normalized using a BCA protein quantitation kit (Thermo Fisher) and analyzed by Western blots using primary antibodies. .. Total RNA was extracted from α‐syn HEK293 cells or primary neurons using the TRIzol reagent (Invitrogen, 15,596,026).

    Article Title: Development of an Indirect ELISA for REV gp90 Antibody Detection Using the gp90 Protein Expressed in Suspended Cells.
    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit anti-chicken immunoglobulin G (IgG) and fluorescein isothiocyanate (FITC)- conjugated rabbit anti-chicken IgG secondary antibodies were purchased from Sigma (Livonia, MI, USA). .. Polyjet in vitro DNA transfection reagent (Signagen, Frederick, MD, USA); Polyethyleneimine (PEI) (Beyotime, Shanghai, China); DMEM liquid culture medium, 293pro CD 293M serum-free culture medium (Shanghai Yuanpei Biotechnology Co., Ltd., Shanghai, China); fetal bovine serum, skim milk powder (Sigma, Livonia, MI, USA); Ni Sepharose 6 Fast Flow (GE, Boston, MA, USA); TMB substrate (TIANGEN, Beijing, China); PageRuler protein marker, Pierce BCA protein quantitation kit (Thermo Scientific, Waltham, MA, USA); phosphate-buffered saline (PBS) buffer (powder) (Coolaber, Beijing, China); Tween-20 (Solarbio, Beijing, China). ..

    Article Title: Purified Diets Lacking Fermentable Fiber Reduce Microbial Diversity, Alter Epithelial Transcriptome, and Exacerbate Colitis
    Article Snippet: .. Total protein concentrations were measured using the Pierce BCA Protein Quantitation kit (Thermo Fisher Scientific). .. Capillary Western analyses were performed using a Wes system (Protein Simple, San Jose, CA, USA) according to the manufacturer’s instructions.

    Article Title: Waterpipe Smoke-Induced N6-Methyladenosine Modification Promotes the Progression of Oral Squamous Cell Carcinoma
    Article Snippet: .. The Pierce BCA protein quantitation kit (Thermo Fisher, USA) was used to measure the protein content. ..

    Article Title: The mechanisms by which hypothalamic neuroinflammation induced by neonatal cerebral ischemia-hypoxia leads to decreased thymic function via the HPA axis.
    Article Snippet: .. Following the instructions of the BCA protein quantitation kit (Thermo, USA), the apoptosis-promoting proteins Cleaved-Caspase 3 (WL02117, Wanleibio), Bcl-2 (WL01556, Wanleibio) and Bax (WL01637, Wanleibio) in thymus were detected. .. Autophagic proteins Beclin-1 (66665-1, Proteintech), LC3 II/I (2775S, CST), p-PI3K (ab302958, Abcam), PI3K (mAb4257S, CST), p-Akt (WLP001a, Wanleibio), Akt (mAb4060S, CST), p-mTOR (5536S, CST), mTOR (mAb2983, CST), GR (WL02695, Wanleibio), β-actin (sc-47778, Santa Cruz) and Histone H3 (4499, CST) in thymus were detected.

    Article Title: Non-canonical function of the splicing activator U2AF2 in promoting intron retention in the lncRNAs PURPL and MALAT1
    Article Snippet: For immunoblotting, total cell lysate was prepared using RIPA buffer (Thermo Scientific) containing protease inhibitor cocktail (Roche). .. Protein concentration was determined using the BCA protein quantitation kit (Thermo Scientific). .. Cell lysate was loaded onto SDS-PAGE gel and transferred to a PVDF membrane (Thermo Scientific) using a semi-dry apparatus (Bio-Rad).

    Western Blot:

    Article Title: SYNGR3 Accelerates α‐Synuclein Aggregation and Neurodegeneration in Parkinson's Disease
    Article Snippet: Pellets were washed in 1 mL TBS, centrifuged at 100,000 × g at 4°C for 1 h, subsequently resuspended in 0.25 mL of 2% SDS in TBS, and finally sonicated and centrifuged at 100,000 × g at 4°C for 1 h. Mouse brain tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer (Beyotime, P0013B) supplemented with a protease inhibitor cocktail (Sigma Aldrich, P9599) and phosphatase inhibitor (APExBIO, K1015). .. The homogenates were subsequently centrifuged at 100,000 × g at 4°C for 1.5 h. The pellet was subsequently resuspended in 2% SDS in TBS buffer and centrifuged at 100,000 × g at 4°C for 1 h. Protein levels were normalized using a BCA protein quantitation kit (Thermo Fisher) and analyzed by Western blots using primary antibodies. .. Total RNA was extracted from α‐syn HEK293 cells or primary neurons using the TRIzol reagent (Invitrogen, 15,596,026).

    In Vitro:

    Article Title: Development of an Indirect ELISA for REV gp90 Antibody Detection Using the gp90 Protein Expressed in Suspended Cells.
    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit anti-chicken immunoglobulin G (IgG) and fluorescein isothiocyanate (FITC)- conjugated rabbit anti-chicken IgG secondary antibodies were purchased from Sigma (Livonia, MI, USA). .. Polyjet in vitro DNA transfection reagent (Signagen, Frederick, MD, USA); Polyethyleneimine (PEI) (Beyotime, Shanghai, China); DMEM liquid culture medium, 293pro CD 293M serum-free culture medium (Shanghai Yuanpei Biotechnology Co., Ltd., Shanghai, China); fetal bovine serum, skim milk powder (Sigma, Livonia, MI, USA); Ni Sepharose 6 Fast Flow (GE, Boston, MA, USA); TMB substrate (TIANGEN, Beijing, China); PageRuler protein marker, Pierce BCA protein quantitation kit (Thermo Scientific, Waltham, MA, USA); phosphate-buffered saline (PBS) buffer (powder) (Coolaber, Beijing, China); Tween-20 (Solarbio, Beijing, China). ..

    Transfection:

    Article Title: Development of an Indirect ELISA for REV gp90 Antibody Detection Using the gp90 Protein Expressed in Suspended Cells.
    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit anti-chicken immunoglobulin G (IgG) and fluorescein isothiocyanate (FITC)- conjugated rabbit anti-chicken IgG secondary antibodies were purchased from Sigma (Livonia, MI, USA). .. Polyjet in vitro DNA transfection reagent (Signagen, Frederick, MD, USA); Polyethyleneimine (PEI) (Beyotime, Shanghai, China); DMEM liquid culture medium, 293pro CD 293M serum-free culture medium (Shanghai Yuanpei Biotechnology Co., Ltd., Shanghai, China); fetal bovine serum, skim milk powder (Sigma, Livonia, MI, USA); Ni Sepharose 6 Fast Flow (GE, Boston, MA, USA); TMB substrate (TIANGEN, Beijing, China); PageRuler protein marker, Pierce BCA protein quantitation kit (Thermo Scientific, Waltham, MA, USA); phosphate-buffered saline (PBS) buffer (powder) (Coolaber, Beijing, China); Tween-20 (Solarbio, Beijing, China). ..

    Marker:

    Article Title: Development of an Indirect ELISA for REV gp90 Antibody Detection Using the gp90 Protein Expressed in Suspended Cells.
    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit anti-chicken immunoglobulin G (IgG) and fluorescein isothiocyanate (FITC)- conjugated rabbit anti-chicken IgG secondary antibodies were purchased from Sigma (Livonia, MI, USA). .. Polyjet in vitro DNA transfection reagent (Signagen, Frederick, MD, USA); Polyethyleneimine (PEI) (Beyotime, Shanghai, China); DMEM liquid culture medium, 293pro CD 293M serum-free culture medium (Shanghai Yuanpei Biotechnology Co., Ltd., Shanghai, China); fetal bovine serum, skim milk powder (Sigma, Livonia, MI, USA); Ni Sepharose 6 Fast Flow (GE, Boston, MA, USA); TMB substrate (TIANGEN, Beijing, China); PageRuler protein marker, Pierce BCA protein quantitation kit (Thermo Scientific, Waltham, MA, USA); phosphate-buffered saline (PBS) buffer (powder) (Coolaber, Beijing, China); Tween-20 (Solarbio, Beijing, China). ..

    Saline:

    Article Title: Development of an Indirect ELISA for REV gp90 Antibody Detection Using the gp90 Protein Expressed in Suspended Cells.
    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit anti-chicken immunoglobulin G (IgG) and fluorescein isothiocyanate (FITC)- conjugated rabbit anti-chicken IgG secondary antibodies were purchased from Sigma (Livonia, MI, USA). .. Polyjet in vitro DNA transfection reagent (Signagen, Frederick, MD, USA); Polyethyleneimine (PEI) (Beyotime, Shanghai, China); DMEM liquid culture medium, 293pro CD 293M serum-free culture medium (Shanghai Yuanpei Biotechnology Co., Ltd., Shanghai, China); fetal bovine serum, skim milk powder (Sigma, Livonia, MI, USA); Ni Sepharose 6 Fast Flow (GE, Boston, MA, USA); TMB substrate (TIANGEN, Beijing, China); PageRuler protein marker, Pierce BCA protein quantitation kit (Thermo Scientific, Waltham, MA, USA); phosphate-buffered saline (PBS) buffer (powder) (Coolaber, Beijing, China); Tween-20 (Solarbio, Beijing, China). ..

    Protein Concentration:

    Article Title: Non-canonical function of the splicing activator U2AF2 in promoting intron retention in the lncRNAs PURPL and MALAT1
    Article Snippet: For immunoblotting, total cell lysate was prepared using RIPA buffer (Thermo Scientific) containing protease inhibitor cocktail (Roche). .. Protein concentration was determined using the BCA protein quantitation kit (Thermo Scientific). .. Cell lysate was loaded onto SDS-PAGE gel and transferred to a PVDF membrane (Thermo Scientific) using a semi-dry apparatus (Bio-Rad).



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